Mayana Leaves Essay

I. IntroductionPeople of different cultures oblige gigantic practiced poses, especially those with therapeutic values. They believe limits to fill healing powers. In most parts of our country, m any people have embraced the powers of plants in their day by day lives. Modern medical specialty has downplayed the importance of this conventional knowledge, but now it has begun to live the value of using plants for medicinal purposes. Plants supply the phytochemicals of the healing system necessary by the body. Phytochemicals be secondary metabolites that are scientifically known as the bioactive constituents with therapeutic activity. They are non-nutritive plant chemical that contain protective, disease-preventing compounds. Thorough covering fire and analysis of these medicinal plants whitethorn expose the bioactive comp wizardnts responsible for their healing capacities.This masking piece involves processes manage extraction, purification and characterization of the act ive constituents of pharmaceutical importance. The Philippines takes pride of its abstruse natural resources and its flora offers an abundant source of medicinal plants. Production and work of local plants with phytochemical properties must be given vital importance since on that point is an immense chance that topically produced and less expensive drugs chamberpot be sourced from them. This may too pave the way for the discovery of novel compounds and locally make drugs that can compete in the global market. Mayana (coleus sp. ) is a traditional plant used to dress cuts and bruises, because of this, we have decided to do a phytochemical toping. The project hopes to find in mayana, value as a sensitive tool in modern medicine at the very least, increment the generalknowledge ab break the plant. The extract will be made by preparing a solution of crushed plants in 80% ethyl group alcohol.The leaf and stem extract were screened for the presence of alkaloids, flavonoids and a nthraquinones. The assay was do by the paper disc method acting of assay. Different concent rations of the extract will be assayed against different kinds of bacteria. The screening showed that the stems contained considerable quantities of alkaloids. The results of the assay showed that tradition holds true in the laboratory as the leaf extract strongly showed signs of antibacterial activity. The reversal was true for the stems as they were found out to facilitate bacterial return instead of impeding it. Future researches may include identification of the particular kind of flavonoids, alkaloids and quinones present on the plant and their specific effects. They may besides try to utilize the bacterial activity of the stem to make a mayana stem-based nutrient agar.ObjectivesTo identify the presence of certain phytochemical constituents present in Mayana To screen the major phytochemical constituents and evaluate antibacterial activity tests of leaf extracts of Mayana To take ca re the antibacterial, antifungal, antiprotozoal, cytotoxic, and phytochemical properties of neutral spirits extracts of leaves of MayanaII. Materials and MethodsA. Folkloric SurveyBefore proceeding with our choice of plant, we did a survey to picture the plants that are used by an albularyo or quack doctor in treating his/her patients. A folkloric survey has been prepared by the researchers and the questions used were given by the professor. The folkloric survey prepared aims to determine the a) the plants that the albularyo still uses at present, b) the medicinal purposes of those plants say by the albularyo, c) the part of the plant that is used by the albularyo and d) the proper method of using the plant medicinally and the recommended dosage. We searched for an albularyo in local/rural areas. The friendship that we chose is located in Bataan.B. Phytochemical ScreeningPlant Collection and PreparationWe stack away about 50 grams of mayana leaves from the local or rural area. We washed it with vindicated water and drained off the excess liquid. subsequentlyward, we air dried them for one week or until the leaves are brittle making sure that they are constantly aerated to prevent the accumulation of fungi on the leaves. subsequently air drying, the leaves were cut into small pieces. The small pieces of leaves were soaked for two eld into a 500 ml beaker containing 200 ml ethanol.Plant bloodlineAfter 2 days of soaking the leaves, we extracted the leaves using filtration. We used a glass funnel with a pick up paper that is set(p) on a tripod for easier use. A beaker was used to collect the extract. The leaves were filtered in the funnel with the filter paper. After filtration, we obtained the filtrate in the beaker. The first layer of the liquid was take away through decantation until the extract was obtained and ready for analysis.Identification of Plant Constituents by lithe Layer ChromatographyThe extract that we obtained through decantation was tested for the presence of phytochemicals using liaison layer chromatography. A tender loving care plate was given to us which measures 2 cm x 7 cm. Using a pencil, we marked the tender loving care plate 1 cm from the top and 1 cm from the bottom. And then, we utilize the plant samples in the bottom mark by blotting through the use of a capillary tube. The blot should be at least 2 mm in diameter but not more than 5 mm for it to climb up the tender loving care plate. After, we prepared solving systems to determine the topper solvent system for our plant extract.In the preparation of the solvent systems, we used hexane and ethyl acetate. There were 3 ratios used 91, 73 and 64. Three tender loving care plates with the blotted plant extract were placed inside the developing chambers seamed with filter paper and cover by a petri dish. The chambers were saturated first with 10 ml of the solvent using the 3 ratios to allow the solvent to move up the lining while cover before placing t he blotted TLC plates.After, thethree TLC plates were placed inside the 3 developing chambers and it was kept covered until the solvent climbed up and reached the pep pill mark. At first, ours didnt show any results and we did several tests. Finally, the blotted TLC plate in the 91 ratio traveled through the TLC. And in our test, the 91 ratio was the best solvent system for our sample.After obtaining the best solvent system to be used, we did an different developing chamber with the 10 ml solvent. Just like what we did, we put the 91 ratio (10 ml solvent) in the beaker and lined it with filter paper to saturate the chamber. We were given two TLC plates, the other having three blots and the other having four blots, a total of sevensome blots. The TLC plates were placed in the developing chamber and waited for the solvent to reach the upper mark. There is a visible spotting or separation of the components in our TLC plates and then we proceeded with the spray reagent tests.The TLC p lates were cut into seven pieces with the seven blots. Our professor assigned each group with a spray reagent. After doing the spray reagent tests, we obtained the results in our chromatograms. We obtained for positive results in Phenols, Coumarins, Anthranoids, and Anthraquinones. The results were written in the data sheet provided.IV. Discussion of ResultsIn the mayana leaves extraction, this was the leaves soaked in 200 ml ethanol. From this solution, we obtained the plant extract from the mayana leaves that we used for the phytochemical screening. In the second picture, the blotted TLC plates, this was done by using the capillary tubes to put the extract in the TLC plate. At the third picture, this shows the TLC plates in the developing chamber with the 10 ml solution which was 9 ml hexane and 1 ml ethanol. Lastly, after the spraying of reagents in the TLC plates, we obtained the results above. The positive results that we obtained are Phenols, Coumarins, Anthranoids and Anthr aquinones, respectively.According to our results, mayana plant had no-account spots which indicate a positive result with phenols. Phenols have anti-inflammatory and anti-oxidant properties which is the same as the mayana plant. Phenols also have antiseptic and it may be present in minimal amount. It also gave a blue zone which tells us that it constitutes coumarins. This indicates that it can be used in wounds. It had discolor zone which shows positive result with anthranoids. Anthranoids can be taken when you have constipation which is the same property as mayana. Orange-violet color was obtained in mayana plant which indicates the presence of anthraquinones. Like phenols andanthranoids, it can be used as anti-inflammatory and for constipation. Some scientists have demonstrated that some derivatives have activity in vitro against some parasitic species.V. ConclusionColeus or mayana is a southeastern Asian plant that has brightly colored variated leaves that is popular as a hou se plant. On our phytochemical screening of this plant, we found out that it has positive results in coumarins which can be used in wounds, anthranoids which can be a treatment for constipation, phenols which can used as anti-inflammatory and anti-oxidant and lastly, anthraquinones which can also be used as anti-inflammatory and for constipation.This plant is also normally used as an ornamental plant due to its purple foliage. Mayana can grow in different habitats. It is one of the traditionally used folklore medicine and it is primarily used for pain, sore, swelling and cuts and other instances as adjunct medicament for delayed menstruation. A chemist from the University of the Philippines isolated sterols and triterpenes from leaves of mayana and it exhibited analgesic, anti-inflammatory and antimicrobial activities. some other interesting component of the plant is its high rosmarinic acid content. This compound was note for its biological activities prominent of those are its a nti-inflammatory and anti-oxidant properties.Lastly, we also found out that mayana has its anti-bacterial, anti-fungal, anti-protozoal and cytotoxic extracts of mayana. Therefore, mayana is a herbal medicine that you may use in wounds, headaches, fever and bruises just like what is said in the survey.VI. Referenceshttp//scinet.dost.gov.ph/union/ShowSearchResult.php?s=2&f=&p=&x=&page=&sid=1&id=The+phytochemical+screening+and+microbiological+assay+of+Mayana+(%3Cem%3EColeus+blumei%3C%2Fem%3E)+leaves+and+stems&Mtype=PROJECTS http//www.filipinoherbshealingwonders.filipinovegetarianrecipe.com/mayana.htm http//www.stuartxchange.com/Mayana.html